Guides

Training material, volume landmarks, access instructions, and citation guidance for Eyewire II.

Get Access

Request view access or email support@eyewire.ai to schedule an onboarding meeting.

Get access

Start Training

Begin with the Spelunker tutorial, then work through the proofreading guides.

Watch tutorial

Use Synapses

Read the beginner synapse guide before labeling synaptic sites in the dataset.

Open PDF

Navigating the Volume

These Neuroglancer states point to landmarks and coordinate systems that help orient analysis in the Eyewire II retina volume.

Ca 2+ imaging fields

Functional imaging fields aligned to the volume.

Open

Stratification layers

Layer references for interpreting arbor depth.

Open

OPL outlines

Outer plexiform layer outlines for tissue context.

Open

Dorsoventral axes

Approximate dorsoventral orientation in Neuroglancer.

Open

Tissue outline and max dimensions

Tissue boundaries and volume dimensions at several z levels.

Open

INL / GCL grid annotation

Grid annotation with example cells from the INL and GCL.

Open

Training Path

3

Submit demo edits

Submit ten links in the Eyewire II dataset: seven accidental mergers with Multicut points and three accidental splits with the two objects you would merge. Do not execute the edits.

We will review your links and send personalized feedback. If it takes more than one try, that is okay; you will not be locked out, and there is no limit on the number of attempts.

Citation and Synapse References

Our principles of collaboration help ensure fair credit assignment for community members and the core team. Use these documents when preparing manuscripts, conference abstracts, preprints, or synapse annotations.

Eyewire 2 citation guidelines

How to cite and acknowledge the dataset and community contributions.

PDF

Synapse Guide for Beginners

Basic information on finding and labeling synapses in the dataset.

PDF

Tools & Tech meeting

Shared meeting materials and demos for data access and analysis workflows.

Drive

Contribute Cells

Before adding a cell, make sure it does not already exist in the main or Researcher spreadsheet. Include nucleus or soma coordinates, status, proofreading link when available, proofread IDs, proofreader or cell typer name, and the cell class or type you identified. See the Cells page for spreadsheet links and column definitions.